Calculate Nm From Ng Ul

DNA Concentration Conversion Formula:

\[ nM = \frac{ng/μL \times 10^6}{Length \times 330} \]

Concentration (nM):

Unit Converter ▲

Unit Converter ▼

From:	To:

1. What is DNA Concentration Conversion?

DNA concentration conversion between ng/μL and nM is essential in molecular biology for normalizing DNA amounts in experiments such as PCR, sequencing, and cloning. It accounts for both mass concentration and molarity based on DNA length.

2. How Does the Calculator Work?

The calculator uses the conversion formula:

\[ nM = \frac{ng/μL \times 10^6}{Length \times 330} \]

Where:

\( ng/μL \) — Mass concentration of DNA
\( Length \) — Length of DNA in base pairs (bp)
\( 330 \) — Average molecular weight per base pair (g/mol)
\( 10^6 \) — Conversion factor from ng to g

Explanation: The formula converts mass concentration to molar concentration by considering the molecular weight of DNA, which depends on its length.

3. Importance of Concentration Conversion

Details: Accurate conversion between ng/μL and nM is crucial for ensuring consistent DNA amounts in reactions, optimizing experimental conditions, and comparing results across different studies.

4. Using the Calculator

Tips: Enter DNA concentration in ng/μL and length in base pairs. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why is DNA length important in concentration conversion?
A: Molar concentration depends on the number of molecules, which is determined by both mass and molecular weight (which varies with length).

Q2: What is the 330 g/mol value based on?
A: This represents the average molecular weight of a DNA base pair (sodium salt), including both nucleotides and counterions.

Q3: Does this conversion work for both double-stranded and single-stranded DNA?
A: The formula is primarily designed for double-stranded DNA. For single-stranded DNA, use 330 g/mol per nucleotide instead of per base pair.

Q4: When is nM concentration preferred over ng/μL?
A: nM is preferred when you need to know the number of molecules (e.g., for stoichiometric calculations in reactions), while ng/μL is better for mass-based measurements.

Q5: Are there limitations to this conversion?
A: The conversion assumes average base composition. For sequences with extreme GC content, slight variations in molecular weight may occur.