Centrifuge G-Force Calculator

Centrifuge G-Force Formula:

\[ RCF = (RPM^2 \times r \times 1.118 \times 10^{-5}) \]

Relative Centrifugal Force (RCF):

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1. What is Relative Centrifugal Force?

Relative Centrifugal Force (RCF) is the measurement of the force applied to a sample in a centrifuge. It's expressed in multiples of the earth's gravitational force (g) and provides a standardized way to compare centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

\[ RCF = (RPM^2 \times r \times 1.118 \times 10^{-5}) \]

Where:

\( RPM \) — Revolutions per minute
\( r \) — Radius in centimeters (distance from center to sample)
\( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts rotational speed (RPM) and radius into gravitational force equivalent, allowing standardization of centrifugation protocols.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is crucial for reproducible centrifugation results, protocol standardization across different centrifuges, and ensuring proper separation of samples without damage.

4. Using the Calculator

Tips: Enter RPM (revolutions per minute) and radius in centimeters. Both values must be positive numbers. The radius is measured from the center of rotation to the sample.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of just RPM?
A: RCF provides a standardized measure of force that accounts for both speed and rotor size, ensuring consistent results across different centrifuges.

Q2: How do I measure the radius accurately?
A: Measure from the center of the centrifuge rotor to the middle of the sample container when it's in the horizontal position.

Q3: What are typical RCF values used in laboratories?
A: RCF values range from a few hundred g for gentle separations to over 100,000 g for high-speed ultracentrifugation.

Q4: Does temperature affect RCF calculation?
A: Temperature doesn't affect the RCF calculation itself, but it can affect sample properties and separation efficiency during centrifugation.

Q5: Can I use this calculator for any type of centrifuge?
A: Yes, the formula applies to all centrifuges, but always verify your equipment's specifications and limitations.